This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. com. 0 out of 5 $ 5,999. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M3. Type:Spectrophotometer. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96-. 3) This warranty. Nucleic Acid and Protein Quantitation. Page 28 Chapter 5: Read Modes and Read Types 1. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. with SpectraMax Microplate Readers Introduction Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. The optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. These robust readers have been placed in labs from. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. This pathlength correction is accomplished only with the use of. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD3 reader. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among. We would like to show you a description here but the site won’t allow us. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. The narrow bandwidths used by the model increase measurement accuracy and linearity over a wide. This system includes the ScanLater Western Blot Detection Cartridge, ScanLater Western Blot Kit and image acquisition software powered by SoftMax Pro® Software. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. Order Now. We would like to show you a description here but the site won’t allow us. Compact designs. Download the SpectraMax Mini Reader Plug-in and associated guides. 专为科研检测平台量身打造的灵活且强大的小型微孔板读板机（酶标仪），具备终点法和动力学检测模式，标准配置6块滤光片，覆盖蛋白定量、细胞活力、农药残留、ELISA实验等. 500 Euro/Dollars for a basic filter-based ELISA microplate reader and could go up to over 150. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. Find regional support. Narrow 2nm bandwidths deliver increased measurement accuracy and linearity over the widest range. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. Temperature control. For readers without a cuvette port, these options are not shown in the software. begin reading microplates with seven mouse clicks. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 μL sample volume in a single read. Contact our support team with your questions regarding Molecular Devices products and applications. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. 0200-6263. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Molecular Devices. 313 reference gain check fail. Results are shown in Table 1. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. 314 low lamp level warning. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. With optimized reagents and the industry-leading data acquisition and analysistool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to perfectly. Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. . For biological applications involving small samples, such as purificationThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Detailed specifications of each model reader are reported in Section 7, “Specifications”. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Add to Cart. 315 can't find zero order. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The dual monochromator optics allow. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. In addition, SoftMax Pro Softwareluminescence, and fluorescence microplate readers, and the SpectraMax® Paradigm® Multi-Mode Detection Platform. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Examples: “Press carefully to engage,” “ Do not. The instrument uses a paired monochromator and xenon flash. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Tryptophan, which excites maximally around 270-280 nm and has an emission peak near 350 nm in water, dominates the emission of proteins and is the mostPhysical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. 1. The advanced optical and. 305 reference level saturation. This unlocks the transport slide. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. 5 12. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications. SpectraMax readers are equipped with PathCheck Technology, which measures the pathlength in each well of a microplate and normalizes the absorbance values to a 1-cm pathlength so that they are the same as values obtained in a 1-cm cuvette. SAN JOSE, CA — Molecular Devices, LLC. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. Microplate Readers Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. Prerequisites: SoftMax Pro 7. This is equivalent to having 511 built-in filters,The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. SpectraMax® 190 absorbance plate reader enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. The plate was placed on an shaker for 15 seconds and then incubated for 2 hours at 37°C, 5% CO 2. Figure 1. Unparalleled performance on a personalized platform. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow toFlexStation® 3 Multi-Mode Microplate Reader. microplate reader. Italic and boldface type are used for emphasis. 0 out of 5 $ 6,499. . Promega's ROS-Glo H202 assay can be combined with SpectraMax readers to explore the relationship between. Figure 3. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The cuvette must be in place when you read the plate. Our absorbance plate readers feature our PathCheck Sensor technology and. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. Lower limits of detection for ATP determined using the ATPlitePage 1 ® SPECTRAmax Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0085 Rev. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. . edu) to be added as a trained user in iLab. Microplate readers’ standard detection modes. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. 4 μL DNA standards. Self Service Available. Sale. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. We would like to show you a description here but the site won’t allow us. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. Microplate automation and detection instruments for walk away operation and high quality results. The University of Rouen in Normandy uses the SpectraMax iD3 and. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. 2 only: SpectraMax Mini Update. Other technologies such as imaging. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command. Request a Quote!Introduction. Sensitive fluorescent quantitation of DNA with the Quant-iT. Absorbance (optical density, OD) was read at 570 nm and 605. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. Step 1: Turn on your microplate reader. Lift up the lid over the chamber to insert or remove a cuvette. Operators can conveniently select up to six wavelengths at a time for absorbance detection in the visible wavelength range of 340 nm – 850 nm. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. limit of detection (LLD) for ATP was estimated for each microplate reader using the . Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. This variable pathlength makes it dif Þcult to perform extinction-based assays and confusing to compare results between microplate readers and spectrophotometers. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. 100 fmol 384-well (75 µL) 0. Download PDF versions of the SpectraMax Microplate Reader User Guides. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Avoid losing high signal. Microplate Readers Molecular Devices SpectraMax M2E Microplate Reader. Win7 Manual Spectramax-L or StakMax Driver Installation after Initial Install. Add to Cart. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. The SpectraMax microplate readers can be used to accurately quantify ROS levels using a luminescence-based assay. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. The price range of a reader starts at 2. These lab workhorses are used in drug discovery, research, bioassay. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. If you do not have an iLab account you must first make one: Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). The plate reader optics are tunable for absorbance. SpectraMax ® M2 Multi-Mode Microplate Reader. For biological applications involving small samples,Minimum volume and maximum throughput with 96- or 384-well microplate compatibility. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. microplate reader. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. The plate reader can measure multiple readouts, including UV. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax® Mini reader is user-upgradeable - from two to three modes, providing researchers with a versatile instrument. The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Absorbance reading with SpectraMax® microplate readers and SoftMax® Pro Software enables optical density measurement of samples in microplate or cuvette format. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Turn the reader off by pulling the power cord. Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as ELISA, protein detection, and drug screening assays. Path Check Pathlength Measurement SpectraMax i3x. 5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12The optical system of SpectraMax Plus384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 1000 nm). Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. Temperature control and shaking come as standard. The advanced optical and. In . View the Molecular Devices website. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual. This technology allows for the automated regulation of well absorbance to a cuvette equivalent path length of 1 cm, which translates to. View the Molecular Devices website. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingevery Gemini EM Reader. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. 1) Select the correct plate and the wells that you want to measure. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. 58% for the detection module and 2. SpectraMax® Microplate Readers and SoftMax® Pro Software. SpectraMax i3x Multi-Mode Detection Platform. Dual-Glo luciferase assay system (Promega Cat. The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. 58% for the detection module and 2. M2. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. Contents of wells were pipetted up and down several times to mix. 316 grating motor driver faulty. Nuclear Receptor. The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. Detailed specifications of each model reader are reported in Section 7, “Specifications”. The temperature in the plate chamber is ambient and isothermal. Fluorescence Microplate Readers. Microplate Readers. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Results are shown in Table 1. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. On the back, in the right hand top corner,. The screw remains inside the instrument. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3 allows users to customize workflow to perfectly. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. Microplate Readers Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. Devices’ StakMax® Microplate Stacker in a matter of minutes and . The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The SpectraMax L Luminescence Microplate Reader with the variable-volume injectors option (10 µL to 100 µL) can measure both flash and glow luminescence. 1. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. HTRF-compatible SpectraMax Multi-Mode Readers. San Jose, Calif. The CellTiter-Glo assay from Promega uses luciferase. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. 020 fmol 0. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Unlock your i3/i3x instrument after shipment. Keywords: spectramax mini multi-mode microplate reader, spectramax mini, spectramax mini microplate reader Created Date: 5/25/2022 9:57:03 AMFully Warranty. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. 100 readings per well gives optimal Z’ values for this assay. Italic and boldface type are used for emphasis. Figure 6: Transcreener ADP2 FI assay principle. 5 87. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The budget-friendly SpectraMax® Mini Multi-Mode Microplate Reader maximizes productivity without compromising sensitivity or performance. BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. 76358-624. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. 0 out of 5 $ 2,999-20%. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. Offer Ends In: 18 Days 19: 38: 21. For SpectraMax ABS Plus reader, r 2 = 0. Multimode Microplate Reader. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). e. Step 1: Open the SoftMax Pro IMAP FP protocol. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Transcreener ® ADP. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has launched its SpectraMax® Mini Multi-Mode Microplate. The calculated LOQ values were 0. Example: [Drawer]. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Table of Contents. Open the top cover of the instrument. Highly customizable low volume. flexibility of monochromator-based systems. Most papers they cultured bacteria to OD600>0. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. 20 μL of Component A was added to each well of the microplate. The drawing shows a standard microplate of 15 mm height covered with a lid. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate312 gain calibration failed. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Detailed specifications of each model reader are reported in Section 7, “Specifications”. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. Step 4: Incubate at room temperature for 1 hour, protected from light. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. It also offers a field-upgradeable cellular imaging option and a cooled PMT for low-light sensitivity. Create custom fluorescence assays without having to purchase expensive filter sets. It measures the optical density (OD) of samples in both 96- and 384-well. Click SoftMax Pro Software User Guide. Endpoint, kinetic, spectrum. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Fluorescence Microplate Reader Comparison Chart. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. Both readers feature an 8-channel read head for fast reads and a subset feature, the temperature independent PathCheck sensor that. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. AlphaScreen. . Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Analytical Technologies Group specializes in the maintenance and repair of Molecular Devices microplate readers, including the SpectraMax® and VersaMax™ absorbance readers. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Or, with the reader specifications noted from the Instrument Description section of your reader's. A. View your data quickly using the large touchscreen interface, export your data for analysis in the program of. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The system uses two scanning monochromators to determine the optimal excitation and emission settings. The dual monochromator optics allow. 1. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published0200-6262. DNA and RNA measurements in SpectraMax® microplateSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Find regional support. More Product Information. If it looks fine, then proceed. The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for traditional flash and glow assays. 0 out of 5 $ 5,999. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS. SpectraDrop High-Throughput Screening (HTS) Kit. flat meniscus), but is approx 15 mL. Also see:. Which SpectraMax M Series reader do you need? SpectraMax M Series Multi-Mode Microplate Readers 5 Ordering information VWR cat. SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. The dual monochromator optics allow the widest range of. no. 40266 Email: drugdiscoverytech@lifetech. For the GMP/GLP regulated labs, the systems come equipped with our SoftMax Pro GxP. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 6% for the on-board optics (monochromators). Multimode Microplate Readers. 0 out of 5 $ 9,995 $ 9,495. It was also read on the monochromator-based. The Molecular Devices Gemini XPS Fluorescent Microplate Reader provides an ideal environment to determine the optimal excitation and emission settings for most fluorescent assays. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. 000 Euro/Dollars for a high-end multi-mode microplate reader with several detection modes and the best technology for highest sensitivity and fastest detection. Guides: SpectraMax Mini Installation Guide. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. We would like to show you a description here but the site won’t allow us. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. Microplate reader settings. ¨0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingThe SpectraMax iD5 Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. Add to Cart. The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. The powerful analysis tools in the software generate a standard curve and calculated data. Detailed specifications of each model reader are reported in Section. Fully tested with validations, warranty. . SpectraMax Microplate Readers. The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. Figure 1. Endpoint, kinetic, spectrum. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. Microplate Spectrophotometers. The drawing shows a standard microplate of 15 mm height covered with a lid. The SpectraMax ABS Plus Microplate Reader is a compact and adaptive microplate reader capable of quantitating nucleic acid and protein samples in various formats such as cuvettes or microplates. Learn more about its features, specifications, and applications in this user. Step 1: Turn on your microplate reader. Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. The SpectraMax 190 reader can be integrated with Molecular . With optimized reagents and the industry-leading data acquisition andSpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. For a higher . With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 µL sample volume in a. The plate. In addition, the iD5 reader measures TRF and FP. 2. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. When the user applies a set temperature of 37°C, the top is heated to 37. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. Published Dec 16, 2020 | Updated Dec 16, 2020. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Examples: “Press carefully to engage,” “ Do not press down. Offer Ends In: 2 Days 10: 40: 19. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. keeping with best practices, reader performance should. The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. The Quant-iT PicoGreen dsDNA Assay Kit, when run on a SpectraMax microplate reader with SoftMax Pro Software, is a quick, sensitive detection method for double-stranded DNA. SpectraMax L Microplate Reader This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. It features a modular design that allows users to add or change detection cartridges according to their needs. . Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Other technologies such as imaging. The SpectraMax® i3 is Molecular Devices latest multi-mode microplate reader which delivers several applications including; Ultra-Violet to visible absorbance detection, fluorescence intensity and multi-colour luminescence.